Characterization of the nuclear export signal in the coronavirus infectious bronchitis virus nucleocapsid protein.
J Virol
; 81(8): 4298-304, 2007 Apr.
Article
em En
| MEDLINE
| ID: mdl-17202223
ABSTRACT
The nucleocapsid (N) protein of infectious bronchitis virus (IBV) localizes to the cytoplasm and nucleolus and contains an eight-amino-acid nucleolar retention motif. In this study, a leucine-rich nuclear export signal (NES) (291-LQLDGLHL-298) present in the C-terminal region of the IBV N protein was analyzed by using alanine substitution and deletion mutagenesis to investigate the relative contributions that leucine residues make to nuclear export and where these residues are located on the structure of the IBV N protein. The analysis indicated that Leu296 and Leu298 are required for efficient nuclear export of the protein. Structural information indicated that both of these amino acids are available for interaction with protein complexes involved in this process. However, export of N protein from the nucleus/nucleolus was not inhibited by leptomycin B treatment, indicating that N protein nuclear export is independent of the CRM1-mediated export pathway.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus da Bronquite Infecciosa
/
Proteínas do Nucleocapsídeo
/
Sinais de Exportação Nuclear
Limite:
Animals
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article