NopM and NopD are rhizobial nodulation outer proteins: identification using LC-MALDI and LC-ESI with a monolithic capillary column.
J Proteome Res
; 6(3): 1029-37, 2007 Mar.
Article
em En
| MEDLINE
| ID: mdl-17249710
ABSTRACT
We have explored the potential of commercial polystyrene-divinylbenzene monolithic capillary nanoLC-MS/MS for identifying Sinorhizobium fredii HH103 nodulation outer proteins. Monolithic nanoLC with off-line MALDI-TOF/TOF and on-line ESI-q-oTOF is fast and robust, generating complementary data and offering high-confidence protein identifications from gel bands too weak for successful analysis using traditional approaches. This has allowed identification of two proteins not previously described as being type III-secreted in rhizobia, NopM and NopD.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas da Membrana Bacteriana Externa
/
Proteínas de Bactérias
/
Sinorhizobium fredii
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article