p130CAS is required for netrin signaling and commissural axon guidance.
J Neurosci
; 27(4): 957-68, 2007 Jan 24.
Article
em En
| MEDLINE
| ID: mdl-17251438
ABSTRACT
Netrins are an important family of axon guidance cues. Here, we report that netrin-1 induces tyrosine phosphorylation of p130(CAS) (Crk-associated substrate). Our biochemical studies indicate that p130(CAS) is downstream of the Src family kinases and upstream of the small GTPase Rac1 and Cdc42. Inhibition of p130(CAS) signaling blocks both the neurite outgrowth-promoting activity and the axon attraction activity of netrin-1. p130(CAS) RNA interference inhibits the attraction of commissural axons in the spinal cord by netrin-1 and causes defects in commissural axon projection in the embryo. These results demonstrate that p130(CAS) is a key component in the netrin signal transduction pathway and plays an important role in guiding commissural axons in vivo.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Axônios
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Sistema de Sinalização das MAP Quinases
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Proteínas Supressoras de Tumor
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Proteína Substrato Associada a Crk
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Fatores de Crescimento Neural
Tipo de estudo:
Guideline
Limite:
Animals
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Female
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Humans
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Pregnancy
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article