Supercritical fluid extraction of quinolizidine alkaloids from Sophora flavescens Ait. and purification by high-speed counter-current chromatography.
J Chromatogr A
; 1145(1-2): 123-7, 2007 Mar 23.
Article
em En
| MEDLINE
| ID: mdl-17289059
ABSTRACT
Supercritical fluid extraction (SFE) was used to extract quinolizidine alkaloids from Sophora flavescens Ait. (Kushen). An orthogonal test L(9)(3)(4) including pressure, temperature, flow rate of CO(2) and the amount of modifier was performed to get the optimal conditions. The process was then scaled up by 30 times with a preparative SFE system under 25 MPa, 50 degrees C and a flow rate of CO(2) (2l/min) and the amount of modifier (0.04 ml/min). The crude extracts were separated and purified by high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of chloroform-methanol-2.3 x 10(-2)M NaH(2)PO(4) (27.52012.5, v/v), and the collected fractions were analyzed by high-performance liquid chromatography (HPLC). Three kinds of quinolizidine alkaloids were obtained, yielding 10.02 mg of matrine, 22.07 mg of oxysophocarpine and 79.93 mg of oxymatrine with purities of 95.6, 95.8, 99.6% in one-step separation, respectively.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Quinolizinas
/
Distribuição Contracorrente
/
Sophora
/
Cromatografia com Fluido Supercrítico
/
Alcaloides
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article