Double-modified albumins as a tool for antibody purification.
J Biochem Biophys Methods
; 70(4): 671-3, 2007 Jun 10.
Article
em En
| MEDLINE
| ID: mdl-17324468
ABSTRACT
A general approach for anti-hapten antibody purification utilizing double-modified albumins is presented. Purification is based on simultaneous modification of an albumin with a hapten (e.g. fluorescein) and desthiobiotin. Three distinct albumins (BSA, HSA and ovalbumin) were modified accordingly and evaluated for their ability to purify the anti-fluorescein mAb from a mixture of commercial preparation and an E. coli cell lysate. The recovered mAb was obtained at relatively high purity (88-95%), in a wide range of target concentrations (0.66-0.02 mg/ml) within a total purification time of approximately 20 min. Substantial increase in the contamination background did not affect purity.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Parvalbuminas
/
Albumina Sérica
/
Soroalbumina Bovina
/
Anticorpos
/
Anticorpos Monoclonais
Limite:
Humans
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article