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The rise of [Na(+)] (i) during ischemia and reperfusion in the rat heart-underlying mechanisms.
Williams, Iwan A; Xiao, Xiao-hui; Ju, Yue-kun; Allen, David G.
Afiliação
  • Williams IA; Bosch Institute and School of Medical Sciences, University of Sydney, F13 Sydney, NSW 2006, Australia.
Pflugers Arch ; 454(6): 903-12, 2007 Sep.
Article em En | MEDLINE | ID: mdl-17356886
Intracellular Na(+) concentration ([Na(+)](i)) rises in the heart during ischemia, and on reperfusion, there is a transient rise followed by a return toward control. These changes in [Na(+)](i) contribute to ischemic and reperfusion damage through their effects on Ca(2+) overload. Part of the rise of [Na(+)](i) during ischemia may be caused by increased activity of the cardiac Na(+)/H(+) exchanger (NHE1), activated by the ischemic rise in [H(+)](i). In support of this view, NHE1 inhibitors reduce the [Na(+)](i) rise during ischemia. Another possibility is that the rise of [Na(+)](i) during ischemia is caused by Na(+) influx through channels. We have reexamined these issues by use of two different NHE1 inhibitors, amiloride, and zoniporide, in addition to tetrodotoxin (TTX), which blocks voltage-sensitive Na(+) channels. All three drugs produced cardioprotection after ischemia, but amiloride (100 microM) and TTX (300 nM) prevented the rise in [Na(+)](i) during ischemia, whereas zoniporide (100 nM) did not. Both amiloride and zoniporide prevented the rise of [Na(+)](i) on reperfusion, whereas TTX was without effect. In an attempt to explain these differences, we measured the ability of the three drugs to block Na(+) currents. At the concentrations used, TTX reduced the transient Na(+) current (I (Na)) by 11 +/- 2% while amiloride and zoniporide were without effect. In contrast, TTX largely eliminated the persistent Na(+) current (I (Na,P)) and amiloride was equally effective, whereas zoniporide had a substantially smaller effect reducing I (Na,P) to 41 +/- 8%. These results suggest that part of the effect of NHE1 inhibitors on the [Na(+)](i) during ischemia is by blockade of I (Na,P). The fact that a low concentration of TTX eliminated the rise of [Na(+)](i) during ischemia suggests that I (Na,P) is a major source of Na(+) influx in this model of ischemia.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sódio / Cardiotônicos / Traumatismo por Reperfusão / Bloqueadores dos Canais de Sódio / Miocárdio Limite: Animals Idioma: En Ano de publicação: 2007 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sódio / Cardiotônicos / Traumatismo por Reperfusão / Bloqueadores dos Canais de Sódio / Miocárdio Limite: Animals Idioma: En Ano de publicação: 2007 Tipo de documento: Article