[The distribution of ATPase during the pollen development of Allium cepa L].

ABSTRACT

The distribution of ATPase in pollen of Allium cepa L. was studied using Pb3 precipitation technique during pollen development. Only some ATPase precipitates were located in the nucleus of microspore mother cells (MMC) and a few in its cytoplasm. After meiosis of MMC,many ATPase precipitates appeared in the exine of pollen wall of microspore even it was in tetrad, suggesting that ATPase from tapetum is necessary during pollen wall construction. The intine of pollen wall of microspore was synthesized at its late stage and consisted of cellular material which was from microspore. There were also many ATPase precipitates in intine,and the ATPase came from microspore. Then ATPase precipitates in vegetative cell increased and that in generative cell decreased during the development of 2-cellular pollen,suggesting the differentiation of vigor between both cells. The physiological functions of ATPase in developing pollen of Allium cepa L. were analyzed.