[A rapid method for preparation of plasmid DNA for screening recombinant clones].
Sheng Wu Gong Cheng Xue Bao
; 23(1): 176-8, 2007 Jan.
Article
em Zh
| MEDLINE
| ID: mdl-17366910
ABSTRACT
A simple and rapid method for preparation of plasmid DNA from overnight incubation was introduced. It does not require any additional reagents; the incubation mixture containing recombinant plasmid DNA was just mixed with H2O and phenol/chloroform/isoamyl alcohol in certain ratio. After vortexing and spinning of the mixture, the supernatant could be directly loaded onto agarose gel and analyzed using electrophoresis. The whole preparation requires only 3-5 minutes. So to quickly screen recombinant clones, this method is better compared with traditional methods.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
DNA Bacteriano
/
Escherichia coli
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Idioma:
Zh
Ano de publicação:
2007
Tipo de documento:
Article