Paraffin baiting system for demonstration of growth and biofilm production in Pseudomonas aeruginosa.
Indian J Exp Biol
; 45(3): 258-60, 2007 Mar.
Article
em En
| MEDLINE
| ID: mdl-17373370
ABSTRACT
Pseudomonas aeruginosa is one of the commonest pathogens among the pseudomonads. This organism can grow in minimal nutritional requirements. Because of the ability of pseudomonads to grow on paraffin is not commonly found among other human pathogens and the primary human pathogen being P. aeruginosa, we studied the adaptation of this organism to paraffin baiting system for growth and biofilm formation. Strains were tested for the capacity to use paraffin as the sole source of carbon using Czapek's minimal salt medium. Of the 53 clinical isolates of P. aeruginosa, 20 strains exhibited growth by 24 hrs and 42 strains by 48 hrs. The remaining strains did not show any growth in the paraffin baiting system. The oxidase test with the paraffin baiting system was also performed. This simple and inexpensive method can be used to isolate and demonstrate the biochemical and biofilm forming capacity of the organism.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Parafina
/
Pseudomonas aeruginosa
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article