Role of NF-kappaB in TNF-alpha-induced COX-2 expression in synovial fibroblasts from human TMJ.
J Dent Res
; 86(4): 363-7, 2007 Apr.
Article
em En
| MEDLINE
| ID: mdl-17384033
ABSTRACT
In the temporomandibular joint (TMJ) synovium, cyclo-oxygenase-2 (COX-2) expression has been believed to be directly related to joint pain and synovitis. Here we investigated the role of Nuclear Factor kappaB (NF-kappaB) in the regulation of COX-2 expression in synovial fibroblasts from human TMJ induced by tumor necrosis factor-alpha (TNF-alpha). By reverse-transcriptase/polymerase chain-reaction (RT-PCR) and Western blotting analysis, TNF-alpha induced a dose- and time-dependent increase in COX-2 expression. Electrophoretic mobility shift assay (EMSA) revealed that transient NF-kappaB activation in the COX-2 promoter was triggered by TNF-alpha. In parallel with transient NF-kappaB activation, the rapid translocation of NF-kappaB, particularly the p65 subunit, from the cytoplasm into the nucleus was demonstrated. Pre-treatment with pyrolidine dithiocarbamate (PDTC), one of the NF-kappaB inhibitors, prevented binding to the COX-2 promoter and expression of COX-2 protein in response to TNF-alpha. These findings indicate that activation of NF-kappaB is responsible for TNF-alpha-induced COX-2 expression in synovial fibroblasts from the TMJ.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Líquido Sinovial
/
Transtornos da Articulação Temporomandibular
/
NF-kappa B
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Fator de Necrose Tumoral alfa
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Ciclo-Oxigenase 2
Limite:
Adolescent
/
Adult
/
Humans
/
Middle aged
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article