Isolation, growth, and purification of defective adenovirus deletion mutants.
Methods Mol Med
; 130: 19-28, 2007.
Article
em En
| MEDLINE
| ID: mdl-17401161
ABSTRACT
Defective adenovirus deletion mutants can be grown by complementation in the presence of helper viruses that supply essential functions missing in the deletion mutant. In general, the deletion mutant then must be separated physically from the helper for use in subsequent experiments. This chapter includes suggestions for selection of helper viruses, protocols for the production of stocks by complementation, and procedures for physical separation of deletion mutants from their helpers.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Adenoviridae
/
Vírus Defeituosos
/
Mutação
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article