The effect of the postnatal environment on altered fetal programming of adult vascular function in mice that lack endothelial nitric oxide synthase.
Am J Obstet Gynecol
; 196(4): 354.e1-7, 2007 Apr.
Article
em En
| MEDLINE
| ID: mdl-17403420
ABSTRACT
OBJECTIVE:
The purpose of this study was to investigate vascular reactivity in heterozygous and homozygous offspring with a genetic predisposition for hypertension after postnatal cross-fostering to mothers with the opposite genetic inheritance of the NOS3 knockout allele. STUDYDESIGN:
Homozygous NOS3 knockout (C57BL/6J-NOS3(-/-KO)) and wild-type mice (NOS3(+/+WT)) were bred to obtain heterozygous litters with a paternally derived (NOS3(+/-pat)) or maternally derived (NOS3(+/-mat)) knockout allele. After delivery, heterozygous and homozygous litters were cross-fostered to a mother with the opposite NOS3 gene status. Carotid arteries were placed in a wire myograph for isometric tension recording with the use of contractile and relaxant agents. Statistical analysis with 1-way analysis of variance and Neuman-Keuls post-hoc testing was performed.RESULTS:
Increased sensitivity to phenylephrine and absent relaxation to acetylcholine in NOS3(+/-mat) was reversed with cross-fostering, and vasorelaxation to isoproterenol was increased. Contraction to calcium was increased in the cross-fostered paternally derived and wild-type litters.CONCLUSION:
Postnatal interventions may alter the adult vascular profile favorably that is the result of an abnormal intrauterine environment.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vasoconstrição
/
Artérias Carótidas
/
Óxido Nítrico Sintase
/
Óxido Nítrico Sintase Tipo II
/
Hipertensão
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Pregnancy
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article