Regulation of the pyrimidine biosynthetic pathway in a pyrD knockout mutant of Pseudomonas aeruginosa.
J Basic Microbiol
; 47(2): 165-73, 2007 Apr.
Article
em En
| MEDLINE
| ID: mdl-17440919
ABSTRACT
In research to date, regulation of the pyrimidine biosynthetic pathway at the level of gene expression has not been shown for wild type Pseudomonas aeruginosa. No repression was observed when uracil was added to the growth medium nor was any derepression seen when Pyr(-) auxotrophs were limited for pyrimidines. Here we show that the addition of uracil to Pseudomonas minimal medium influenced the synthesis of pyrimidine enzymes, while starvation of a pyrimidine knockout mutant (pyrD) elicited derepression of the pyrimidine enzymes. Moreover, the inclusion of orotate in the growth medium induced the synthesis of dihydroorotase in both wild type and mutant. These results suggest that the pyrimidine pathway in P. aeruginosa is regulated at the level of enzyme synthesis in a manner similar to a number of other Pseudomonas species.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pseudomonas aeruginosa
/
Pirimidinas
/
Repressão Enzimática
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article