Diagnosis of canine brucellosis by ELISA using an antigen obtained from wild Brucella canis.
Res Vet Sci
; 83(3): 340-6, 2007 Dec.
Article
em En
| MEDLINE
| ID: mdl-17442351
ABSTRACT
An indirect ELISA test was developed for the diagnosis of Brucella canis infection in dogs. A bacterial whole cell extract was used as a solid phase antigen, using B. canis isolated from an infected animal. Sera from culture-positive and healthy negative animals were used as internal reference controls. The cut-off point was determined by a mathematical formula for a statistically valid value, which defined the upper prediction limit, based on the upper tail of the t-distribution of 21 negative control sera readings, for the confidence level of 99.5%. The sensitivity and specificity of the ELISA test were 95% and 91%, respectively. The ELISA test showed a significant concordance index (K=0.84) with the agar gel immunodiffusion test. The reliability of the ELISA for the detection of infected animals was established by a double blind study testing 280 sera provided by serum banks from different diagnostic and research institutions and analyzed by ROC Curve.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Brucelose
/
Ensaio de Imunoadsorção Enzimática
/
Brucella canis
/
Doenças do Cão
/
Antígenos de Bactérias
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article