Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science
; 316(5831): 1625-8, 2007 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-17569867
ABSTRACT
Genome sequences for most metazoans and plants are incomplete because of the presence of repeated DNA in the heterochromatin. The heterochromatic regions of Drosophila melanogaster contain 20 million bases (Mb) of sequence amenable to mapping, sequence assembly, and finishing. We describe the generation of 15 Mb of finished or improved heterochromatic sequence with the use of available clone resources and assembly methods. We also constructed a bacterial artificial chromosome-based physical map that spans 13 Mb of the pericentromeric heterochromatin and a cytogenetic map that positions 11 Mb in specific chromosomal locations. We have approached a complete assembly and mapping of the nonsatellite component of Drosophila heterochromatin. The strategy we describe is also applicable to generating substantially more information about heterochromatin in other species, including humans.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Heterocromatina
/
Análise de Sequência de DNA
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Drosophila melanogaster
Limite:
Animals
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article