Early gene expression profiles during intraoperative myocardial ischemia-reperfusion in cardiac surgery.
J Thorac Cardiovasc Surg
; 134(1): 74-81, 81.e1-2, 2007 Jul.
Article
em En
| MEDLINE
| ID: mdl-17599489
ABSTRACT
OBJECTIVE:
The effects of cold cardioplegic arrest and reperfusion on human ventricular gene expression are unknown. We tested the hypothesis that intraoperative ischemia-reperfusion under conditions of blood cardioplegic arrest would induce a unique myocardial genomic profile indicative of a cardioprotective response.METHODS:
Right ventricular samples were serially acquired during surgical repair of ventricular septal defect.RESULTS:
Expression profiling revealed 3 patterns of gene expression (1) increased expression above control levels within 1 hour of cardioplegic arrest, with further amplification during early reperfusion; (2) increased expression limited to the reperfusion phase; and (3) reduced expression during reperfusion. Functional annotation and network mapping of differentially expressed genes indicated activation of multiple signaling pathways regulated by phosphatidylinositide 3'-OH kinase convergent on cellular growth and reparative programs. Also observed was increased expression of genes regulating hemoglobin synthesis, suggesting a novel cardioprotective pathway evoked during ischemia-reperfusion.CONCLUSION:
Reversible myocardial ischemia-reperfusion during cardiac surgery is associated with an immediate genomic response that predicts a net cardioprotective phenotype.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Reperfusão Miocárdica
/
Isquemia Miocárdica
/
Perfilação da Expressão Gênica
/
Comunicação Interventricular
/
Miocárdio
Tipo de estudo:
Etiology_studies
/
Prognostic_studies
Limite:
Child, preschool
/
Humans
/
Infant
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article