Subcloning Dt40 by limiting dilution.
Subcell Biochem
; 40: 393-4, 2006.
Article
em En
| MEDLINE
| ID: mdl-17623927
ABSTRACT
Subcloning by limited dilution can be used to derive clonally related cell populations from a heterogeneous DT40 cell culture. For example, if one suspects that a drug resistant population may represent the progeny of more than one transfectant, the protocol can be used to isolate genetically homogeneous mutant clones. Other uses are the excision of floxed DNA sequences after Cre recombinase expression or fluctuation analysis to determine mutation rates (see Protocols 'Excision of floxed-DNA sequences by transient induction of Mer-Cre-Mer' and 'Analysis of sIgM expression by FACS').
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfócitos B
/
Células Clonais
Limite:
Animals
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article