Immunogenicity and protection against genital Chlamydia infection and its complications by a multisubunit candidate vaccine.
J Microbiol Immunol Infect
; 40(3): 188-200, 2007 Jun.
Article
em En
| MEDLINE
| ID: mdl-17639158
ABSTRACT
BACKGROUND AND PURPOSE:
Genital infections due to Chlamydia trachomatis pose a considerable public health challenge worldwide and a vaccine is urgently needed to protect against these infections. We examined whether a vaccine composed of a combination of the major outer membrane protein (MOMP) and porin B protein (PorB) of C. trachomatis would have a protective advantage over a single subunit construct.METHODS:
Single and multisubunit vaccines expressing MOMP and PorB were constructed and evaluated in the mouse model of genital infection. Thus, groups of female C57BL/6 mice were immunized intramuscularly with recombinant Vibrio cholerae ghosts (VCG) expressing the vaccine antigens or VCG alone and humoral and cell-mediated immune responses were evaluated.RESULTS:
Significant levels of Chlamydia-specific secretory immunoglobulin A and immunoglobulin G2a were detected in vaginal washes and serum of immunized mice. The multisubunit construct induced a significantly higher level of T-helper Type 1 response than the single subunits as measured by the amount of interferon-gamma produced by immune T cells in response to re-stimulation with ultraviolet-irradiated elementary bodies in vitro. Three weeks after the last immunization, animals were challenged intravaginally with 10(7) inclusion-forming units of C. trachomatis serovar D. There was a significant difference in the intensity and duration of vaginal shedding between the vaccine-immunized mice and controls. All the animals immunized with the multisubunit vaccine had completely resolved the infection 2 weeks post-challenge. Higher numbers of embryos were observed in vaccinated animals than in controls, indicating protection against infertility.CONCLUSION:
These results underscore the potential, albeit moderate, vaccine advantage of the multisubunit formulation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas da Membrana Bacteriana Externa
/
Proteínas de Bactérias
/
Doenças Vaginais
/
Vacinas Bacterianas
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Infecções por Chlamydia
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Chlamydia trachomatis
/
Porinas
Limite:
Animals
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article