The nuclear coded mitoribosomal proteins YmL27 and YmL31 are both essential for mitochondrial function in yeast.
Biochimie
; 73(6): 837-44, 1991 Jun.
Article
em En
| MEDLINE
| ID: mdl-1764528
ABSTRACT
Using synthetic oligonucleotides deduced from the N-terminal amino acid sequence of purified mitoribosomal protein (mt r-protein) YmL27, the corresponding nuclear gene MRP-L27 of the yeast Saccharomyces cerevisiae has been cloned and sequenced. The MRP-L27 gene codes for 146 amino acids and is located on chromosome X. The mature YmL27 protein consists of 130 amino acids - after cleaving the putative mitochondrial signal peptide - with a net charge of +17 and a calculated relative molecular mass of 14,798 Da. The YmL27 protein as well as the yeast mitoribosomal protein YmL31, which had been characterized and its gene (MRP-L31) cloned previously, is essential for mitochondrial function as shown by the inability of gene disrupted mutants for the MRP-L27 or MRP-L31 genes to grow on non-fermentable carbon sources.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Ribossômicas
/
Saccharomyces cerevisiae
/
Núcleo Celular
/
Mapeamento Cromossômico
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Mitocôndrias
Idioma:
En
Ano de publicação:
1991
Tipo de documento:
Article