Isolation and molecular characterization of a cryptic plasmid from Bifidobacterium longum.
Biotechnol Lett
; 30(1): 145-51, 2008 Jan.
Article
em En
| MEDLINE
| ID: mdl-17700996
ABSTRACT
An isolate from the fecal samples of children was identified as Bifidobacterium longum. A plasmid isolated from it pBIFA24 was 4,892 bp with three open reading frames, ORFI, ORFII, and ORFIII. ORFI encoded a replication protein involved in a rolling-circle replication mechanism, and three sets of tandem repeat sequences featuring iteron structure were identified. Secondary structure prediction analysis of ORFII suggested it was a transmembrane protein. ORFIII showed high amino acid sequence identity with some mobilization proteins and contained an oriT sequence.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Bifidobacterium
/
DNA Bacteriano
/
Sítios de Splice de RNA
/
Fezes
Limite:
Child
/
Humans
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article