Endogenous cyclic AMP in thyroid cell culture. Effect of thyroid-stimulating hormone and dibutyryl cyclic AMP.
Biochim Biophys Acta
; 428(1): 233-9, 1976 Mar 25.
Article
em En
| MEDLINE
| ID: mdl-177060
ABSTRACT
We have studied the variations of endogenous cyclic AMP levels in thyroid cells cultured over a period of 7 days in several conditions in the presence of thyroid-stimulating hormone or dibutyryl cyclic AMP which both promote the aggregation of isolated cells into follicles, and in their absence when cells develop as a typical monolayer. In follicle-forming cells, the cyclic AMP level was found to rise during the first day of culture, then to fall rapidly. In monolayer-forming cells, the cyclic AMP content slightly increases attaining the same level as found in other cells at the fourth day, which remains stable till the seventh day. We have investigated the response of these cells cultured in the presence of dibutyryl cyclic AMP retain the capability of increasing their cyclic AMP concentration whereas monolayer-forming cells do not preserve this quality of thyroid cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Glândula Tireoide
/
Tireotropina
/
AMP Cíclico
/
Bucladesina
Limite:
Animals
Idioma:
En
Ano de publicação:
1976
Tipo de documento:
Article