An activity-independent selection system of thermostable protein variants.
Nat Methods
; 4(11): 919-21, 2007 Nov.
Article
em En
| MEDLINE
| ID: mdl-17906633
ABSTRACT
We describe an activity-independent method for the selection of thermostable mutants of any protein. It is based on a fusion construct comprising the protein of interest and a thermostable antibiotic resistance reporter, in such a way that thermostable mutants provide increased resistance in a thermophile. We isolated thermostable mutants of three human interferons and of two enzymes to demonstrate the applicability of the system.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Engenharia de Proteínas
/
Proteínas
/
Temperatura Alta
Limite:
Humans
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article