Isolation of genes expressed during the developmental stages of the oyster mushroom, Pleurotus ostreatus, using expressed sequence tags.
FEMS Microbiol Lett
; 276(1): 19-25, 2007 Nov.
Article
em En
| MEDLINE
| ID: mdl-17937661
ABSTRACT
In order to isolate and identify the developmentally regulated genes during fruiting body development, cDNA libraries were constructed from eight developmental stages of the Oyster mushroom, Pleurotus ostreatus. From these libraries, 11 761 expressed sequence tags (PoESTs) were generated. Of these, 4060 different genes (PoUnigenes) were identified, representing 34.5% of the entire genome. Redundancy analysis of ESTs during the developmental stages identified eight, 13 and two genes that were specifically expressed in mycelia, fruiting body and basidiospore, respectively. RT-PCR was used to confirm the specific expression of nine genes which showed specific redundancy in fruiting body stages, four genes of which were expressed specifically in fruiting body stages as expected in redundancy analysis, and other genes were expressed abundantly in fruiting body stages. The EST database of P. ostreatus generated during this study provides a genetic and biochemical basis for future studies of the developmental stages of basidiomycetes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Regulação Fúngica da Expressão Gênica
/
Pleurotus
/
Etiquetas de Sequências Expressas
/
Perfilação da Expressão Gênica
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article