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Biomolecular interaction monitoring of autoantibodies by scanning surface plasmon resonance microarray imaging.
Lokate, Angelique M C; Beusink, J Bianca; Besselink, Geert A J; Pruijn, Ger J M; Schasfoort, Richard B M.
Afiliação
  • Lokate AM; Department of Biomolecular Chemistry, Nijmegen Centre for Molecular Life Sciences, Institute for Molecules and Materials, Radboud University Nijmegen, P. O. Box 9101, NL-6500 HB Nijmegen, The Netherlands.
J Am Chem Soc ; 129(45): 14013-8, 2007 Nov 14.
Article em En | MEDLINE | ID: mdl-17941638
A new commercial surface plasmon resonance (SPR) imaging analysis system with a novel SPR dip angle scanning principle allows the measurement, without the need for labeling, of the exact SPR dip angle. With this system hundreds of biomolecular interactions can be monitored on microarrays simultaneously and with great precision. The potency of this system is demonstrated by automatically monitoring the interactions between citrullinated peptides and serum autoantibodies of 50 rheumatoid arthritis (RA) patients and 29 controls in a single step. The smallest antibody concentration that could be measured in this experimental setup was 0.5 pM.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / Autoanticorpos / Aumento da Imagem / Ressonância de Plasmônio de Superfície / Análise Serial de Proteínas Limite: Humans Idioma: En Ano de publicação: 2007 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / Autoanticorpos / Aumento da Imagem / Ressonância de Plasmônio de Superfície / Análise Serial de Proteínas Limite: Humans Idioma: En Ano de publicação: 2007 Tipo de documento: Article