Immunochemical and immunohistochemical studies on distribution of elastin fibres in human atherosclerotic lesions using a polyclonal antibody to elastin-derived hexapeptide repeat.
J Biochem
; 142(5): 627-31, 2007 Nov.
Article
em En
| MEDLINE
| ID: mdl-17951286
ABSTRACT
A polyclonal antibody to elastin-derived hexapeptide repeat, H-(Val-Gly-Val-Ala-Pro-Gly)(3)-NH(2), was prepared in order to investigate the differences between elastin fibres in intimal hyperplasia and media in human atheroscleroic lesions. The hexapeptide repeat and alpha-elastin were recognized by this polyclonal antibody in enzyme-linked immunosorbent assay (ELISA), but other elastin-derived peptides such as tetrapeptide repeat, pentapeptide repeat and nonapeptide were not. In the series of hexapeptide repeats, H-(VGVAPG)(n)-NH(2) where n is 1-7, the polyclonal antibody reacted strongly with oligomers (n = 3-7) and weakly with dimer (n = 2), but not with monomer (n = 1). CD measurements suggested that the beta-turn structure is important for recognition by the polyclonal antibody. In an immunohistochemical study, elastin was stained more strongly in intimal hyperplasia than in media, suggesting that newly synthesized elastin in intimal hyperplasia is morphologically distinct from that in media.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligopeptídeos
/
Elastina
/
Tecido Elástico
/
Aterosclerose
/
Anticorpos
Limite:
Humans
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article