Top-down mass spectrometry on low-resolution instruments: characterization of phosphopantetheinylated carrier domains in polyketide and non-ribosomal biosynthetic pathways.
Bioorg Med Chem Lett
; 18(10): 3107-11, 2008 May 15.
Article
em En
| MEDLINE
| ID: mdl-18006314
ABSTRACT
Mass spectrometry (MS) is an important tool for studying non-ribosomal peptide, polyketide, and fatty acid biosynthesis. Here we describe a new approach using multi-stage tandem MS on a common ion trap instrument to obtain high-resolution measurements of the masses of substrates and intermediates bound to phosphopantetheinylated (holo) carrier proteins. In particular, we report the chemical formulas of 12 diagnostic MS(3) fragments of the phosphopantetheine moiety ejected from holo carrier proteins during MS(2). We demonstrate our method by observing the formation of holo-AcpC, a putative acyl carrier protein from Streptococcus agalactiae.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Panteteína
/
Peptídeo Sintases
/
Proteína de Transporte de Acila
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Transdução de Sinais
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Macrolídeos
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article