Riboswitch-dependent gene regulation and its evolution in the plant kingdom.
Genes Dev
; 21(22): 2874-9, 2007 Nov 15.
Article
em En
| MEDLINE
| ID: mdl-18006684
ABSTRACT
Riboswitches are natural RNA sensors that affect gene control via their capacity to bind small molecules. Their prevalence in higher eukaryotes is unclear. We discovered a post-transcriptional mechanism in plants that uses a riboswitch to control a metabolic feedback loop through differential processing of the precursor RNA 3' terminus. When cellular thiamin pyrophosphate (TPP) levels rise, metabolite sensing by the riboswitch located in TPP biosynthesis genes directs formation of an unstable splicing product, and consequently TPP levels drop. When transformed in plants, engineered TPP riboswitches can act autonomously to modulate gene expression. In an evolutionary perspective, a TPP riboswitch is also present in ancient plant taxa, suggesting that this mechanism is active since vascular plants emerged 400 million years ago.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plantas
/
RNA de Plantas
/
Regulação da Expressão Gênica de Plantas
/
Evolução Molecular
Tipo de estudo:
Risk_factors_studies
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article