Fully automated four-column capillary LC-MS system for maximizing throughput in proteomic analyses.
Anal Chem
; 80(1): 294-302, 2008 Jan 01.
Article
em En
| MEDLINE
| ID: mdl-18044960
ABSTRACT
We describe a four-column, high-pressure capillary liquid chromatography (LC) system for robust, high-throughput liquid chromatography-mass spectrometry (LC-MS(/MS)) analyses. This system performs multiple LC separations in parallel, but staggers each of them such that the data-rich region of each separation is sampled sequentially. By allowing nearly continuous data acquisition, this design maximizes the use of the mass spectrometer. Each analytical column is connected to a corresponding ESI emitter in order to avoid the use of postcolumn switching and associated dead volume issues. Encoding translation stages are employed to sequentially position the emitters at the MS inlet. The high reproducibility of this system is demonstrated using consecutive analyses of global tryptic digest of the microbe Shewanella oneidensis.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas
/
Cromatografia Líquida de Alta Pressão
/
Proteômica
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article