The use of ELISA for the detection of African horse sickness viruses in Culicoides midges.
J Virol Methods
; 34(2): 221-5, 1991.
Article
em En
| MEDLINE
| ID: mdl-1804852
ABSTRACT
The use of ELISA for the detection of African horse sickness viruses (AHSV) in midges preserved in 5.0% formalin was evaluated. No differences were detected by ELISA when testing AHSV infected batches of Culicoides midges collected in diluent with or without the addition of formalin. The ELISA was considered highly sensitive and easily distinguished between non-infected midges and batches containing varying numbers of infected and non-infected midges. Positive ELISA reactions were detected with formalin-preserved midges collected from the south of Spain during the 1988 AHSV epizootic. The assay, therefore, may be used in surveillance studies of either fresh or formalin-preserved midges to identify undisclosed and persistent AHSV foci. This information would be useful in helping to eradicate the virus from Europe and North Africa.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ensaio de Imunoadsorção Enzimática
/
Ceratopogonidae
/
Doença Equina Africana
Tipo de estudo:
Diagnostic_studies
/
Observational_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
1991
Tipo de documento:
Article