[Early diagnosis of bladder cancer by special fluorescence spectrum: an in vitro experiment].
Zhonghua Yi Xue Za Zhi
; 87(36): 2544-6, 2007 Sep 25.
Article
em Zh
| MEDLINE
| ID: mdl-18067829
ABSTRACT
OBJECTIVE:
To investigate the value of special fluorescence spectrum in the early diagnosis of bladder cancer.METHODS:
Hematoporphyrin monomethyl ether (HMME) of the concentrations of 10, 100, and 200 microg/ml, as photosensitizer, was added into the sample vessels of bladder cancer tissue to be incubated for 1 - 3 h. A detecting system consisting of solid state ultraviolet laser with the wavelength of 355 nm was used to measure the fluorescence spectrum.RESULTS:
The laser-induced auto-fluorescence (LIAF) of the bladder cancer tissue had characteristic double peaks at 445 - 490 nm. There was a remarkable characteristic peak, drug peak, located at 628.25 nm in the laser-induced drug-fluorescence (LIDF) of the bladder cancer tissue soaked by HMME.CONCLUSION:
Photosentisizer raise the sensitivity and specificity of fluorescence spectrum in diagnosis of bladder cancer. Stable, and with high strength and visible drug peak, the LIDF is may help improve the early diagnosis of bladder cancer.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Fluorescência
/
Neoplasias da Bexiga Urinária
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2007
Tipo de documento:
Article