Differentiation of mouse embryonic stem cells and of human adult stem cells into adipocytes.
Curr Protoc Cell Biol
; Chapter 23: Unit 23.4, 2007 Mar.
Article
em En
| MEDLINE
| ID: mdl-18228505
ABSTRACT
The authors describe protocols for culture conditions in which mouse ES cells can be maintained in an undifferentiated state or committed to undergo adipocyte differentiation at a high rate and in a highly reproducible fashion. There is also a protocol for maintaining and differentiating human adult stem cells, isolated form adipose tissue and from bone marrow, into adipocytes. These culture systems provide a powerful means for studying the first step of adipose cell development and a means to investigate effects of drugs on the biology of adipocytes. There are also protocols for detection of adipocytes and analysis of their gene expression.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Adipócitos
/
Técnicas de Cultura de Células
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Células-Tronco Multipotentes
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Células-Tronco Adultas
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Células-Tronco Embrionárias
Limite:
Adult
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Animals
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Humans
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article