Anti-apoptotic role of S100A8 in X-ray irradiated keratinocytes.
J Dermatol Sci
; 51(1): 11-8, 2008 Jul.
Article
em En
| MEDLINE
| ID: mdl-18325741
ABSTRACT
BACKGROUND:
Ionizing radiation is used to treat a lot of cancers, however, it also produced unwanted side effect on normal tissues, such as radiodermatitis. We previously established an animal model for radiodermatitis, and identified many of radiation-induced genes by cDNA microarray. Of the candidates, we chose S100A8 gene for a further study.OBJECTIVE:
The aim of this study is to investigate the functional role of S100A8 in X-ray irradiated keratinocytes.METHODS:
RT-PCR and immunohistochemistry were performed to demonstrate the S100A8 induction by X-ray irradiation. HaCaT keratinocytes were transduced with the recombinant adenovirus expressing GFP-S100A8, and then effects on cell cycle and apoptosis were analyzed using flow cytometry and Western blot.RESULTS:
X-ray irradiation markedly induced S100A8 expression in the hyperplastic epidermis of mouse. Overexpression of S100A8 by adenoviral transduction led to the enhancement of cell proliferation in the absence and/or presence of X-ray irradiation, as compared with Ad/GFP control group. Furthermore, overexpression of S100A8 significantly protected the X-ray-induced apoptosis.CONCLUSION:
These results suggest that S100A8 have an anti-apoptotic role in X-ray irradiated keratinocytes.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Queratinócitos
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Calgranulina A
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Proliferação de Células
Limite:
Animals
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Humans
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Male
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article