DeltaNp63/BMP-7-dependent expression of matrilin-2 is involved in keratinocyte migration in response to wounding.
Biochem Biophys Res Commun
; 369(4): 994-1000, 2008 May 16.
Article
em En
| MEDLINE
| ID: mdl-18328806
ABSTRACT
p63 is expressed as multiple variants including TA and DeltaN forms. Since p63-deficient mice displayed profound defects of stratified epithelia, p63 is an essential transcription factor required for epidermal morphogenesis. However, precise molecular mechanisms behind contribution of p63 to normal skin formation and healing skin wounds remained unclear. In this study, we demonstrated that DeltaNp63/BMP-7 signaling pathway modulates wound healing process through the regulation of extracellular matrix protein matrilin-2. Knocking down of DeltaNp63 in human keratinocyte HaCaT cells led to a significant reduction of matrilin-2. Intriguingly, BMP-7 which is one of DeltaNp63-target gene products, induced matrilin-2 and attenuated inhibitory effect of siRNA against DeltaNp63 on matrilin-2. Furthermore, a remarkable cell migration in response to wounding took place in DeltaNp63- or matrilin-2-knocked down cells. Taken together, our present findings indicate that DeltaNp63/BMP-7 signaling pathway modulates wound healing process through the regulation of matrilin-2.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cicatrização
/
Glicoproteínas
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Queratinócitos
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Transativadores
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Movimento Celular
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Regulação da Expressão Gênica
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Proteínas da Matriz Extracelular
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Fator de Crescimento Transformador beta
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Proteínas Morfogenéticas Ósseas
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Proteínas Supressoras de Tumor
Limite:
Humans
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article