Direct recovery of infectious pestivirus from a full-length RT-PCR amplicon.
J Virol Methods
; 149(2): 330-3, 2008 May.
Article
em En
| MEDLINE
| ID: mdl-18336923
ABSTRACT
This study describes the use of a novel and rapid long reverse transcription (RT)-PCR for the generation of infectious full-length cDNA of pestiviruses. To produce rescued viruses, full-length RT-PCR amplicons of 12.3 kb, including a T7-promotor, were transcribed directly in vitro, and the resulting RNA transcripts were electroporated into ovine cells. Infectious virus was obtained after one cell culture passage. The rescued viruses had a phenotype similar to the parental Border Disease virus strain. Therefore, direct generation of infectious pestiviruses from full-length RT-PCR cDNA products could be a valuable instrument for virus rescue, conservation and further characterization.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Genoma Viral
/
Vírus da Doença da Fronteira
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Limite:
Animals
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article