In vitro analysis of translation enhancers.
Methods Mol Biol
; 451: 113-24, 2008.
Article
em En
| MEDLINE
| ID: mdl-18370251
ABSTRACT
The genomes of many plant viruses contain translation-enhancing sequences that allow them to compete successfully with host messenger RNAs for the translation machinery. Identification of translation enhancer elements is valuable, both to gain understanding of virus gene expression control and to apply them as tools for engineering gene expression in plant cells. Here, we describe experiments designed to detect viral elements that enhance translation, focusing on cap-independent translation activity, using a high fidelity cell-free wheat germ translation extract.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus de Plantas
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Biossíntese de Proteínas
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RNA Mensageiro
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Técnicas Genéticas
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Elementos Facilitadores Genéticos
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Genoma Viral
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article