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Azaspiracid: first evidence of protein binding in shellfish.
Nzoughet, K J; Hamilton, J T G; Floyd, S D; Douglas, A; Nelson, J; Devine, L; Elliott, C T.
Afiliação
  • Nzoughet KJ; Institute of Agri-food and Land Use, School of Biological Sciences, Queen's University Belfast, David Keir Building, Stranmillis Road, Belfast BT9 5AG, Northern Ireland, UK. ahou205@yahoo.fr
Toxicon ; 51(7): 1255-63, 2008 Jun 01.
Article em En | MEDLINE | ID: mdl-18417183
The occurrence of azaspiracid (AZA) toxins in contaminated shellfish has been the focus of much research. The present study investigated the binding properties of these toxins in mussels of the species Mytilus edulis. The work involved extraction of proteins and AZAs from contaminated mussel hepatopancreas and examination of the extracts by isoelectric focusing (IEF), size exclusion chromatography (SEC) and sodium docecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Liquid chromatography coupled with tandem mass spectrometry analysis (LC-MS/MS) was also performed in this study to identify AZAs. Blank mussels were subjected to the same purification and analytical procedures. AZAs were found to be weakly bound to a protein with a molecular weight of 45 kDa, in samples of contaminated mussels. This protein, which was abundant in contaminated mussels, was also present in blank mussels, albeit at much lower concentrations. It was further noted that a 22 kDa protein was also present only in contaminated mussel samples.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Frutos do Mar / Compostos de Espiro / Proteínas / Contaminação de Alimentos / Mytilus edulis / Doenças Transmitidas por Alimentos / Toxinas Marinhas Limite: Animals Idioma: En Ano de publicação: 2008 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Frutos do Mar / Compostos de Espiro / Proteínas / Contaminação de Alimentos / Mytilus edulis / Doenças Transmitidas por Alimentos / Toxinas Marinhas Limite: Animals Idioma: En Ano de publicação: 2008 Tipo de documento: Article