In vivo transfer of plasmid from food-grade transiting lactococci to murine epithelial cells.
Gene Ther
; 15(16): 1184-90, 2008 Aug.
Article
em En
| MEDLINE
| ID: mdl-18418419
ABSTRACT
We recently demonstrated that noninvasive food-grade Lactococcus lactis (L. lactis) can deliver eukaryotic expression plasmid in mammalian cells in vitro. Here, we evaluated, in vivo, whether a eukaryotic expression plasmid carried by lactococci can translocate to the epithelial cells of the intestinal membrane. The strain LL(pLIGBLG1) carrying one plasmid containing a eukaryotic expression cassette encoding beta-lactoglobulin (BLG), a major allergen of cow's milk, was orally administered by gavage to mice. BLG cDNA was detected in the epithelial membrane of the small intestine of 40% of the mice and BLG was produced in 53% of the mice. Oral administration of LL(pLIGBLG1) induced a low and transitory Th1-type immune response counteracting a Th2 response in case of further sensitization. We demonstrated for the first time the transfer of a functional plasmid to the epithelial membrane of the small intestine in mice by noninvasive food-grade lactococci.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Terapia Genética
/
Hipersensibilidade a Leite
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Lactococcus lactis
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Translocação Bacteriana
Limite:
Animals
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article