Enzymic thin film coatings for bioactive materials.
Biotechnol Prog
; 24(3): 714-9, 2008.
Article
em En
| MEDLINE
| ID: mdl-18429640
ABSTRACT
Bioactive materials have been explored for a broad range of applications including biocatalysts, biosensors, antifouling membranes and other functional and smart materials. We report herein a unique method for preparation of bioactive materials through a spin coating process. Specifically, we investigated the preparation of protease Subtilisin Carlsberg-coated plastic films and examined their activities for hydrolysis of chicken egg albumin (CEA). The process generated enzymic coatings with a typical loading of 13 microg/cm2, retaining 46% of the enzyme activity for hydrolysis of CEA in aqueous solutions. Interestingly, the surface-coated protease thin film not only catalyzed the hydrolysis of CEA in aqueous solutions, but also showed good activity for solid-state CEA that was coated on top of the enzyme thin film.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plásticos
/
Materiais Revestidos Biocompatíveis
/
Subtilisina
/
Membranas Artificiais
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article