Methods to assay inhibitors of tRNA synthetase activity.
Methods Mol Med
; 142: 53-61, 2008.
Article
em En
| MEDLINE
| ID: mdl-18437305
ABSTRACT
Aminoacyl-tRNA synthetases (aa-RS) attracted interest as potential targets for new antibacterial compounds. Most organisms express 20 aa-RSs one for each amino acid. Aa-RSs are essential proteins in all living organisms. When one aa-RS is inhibited, the corresponding tRNA is not charged and is therefore unavailable for translation. This leads to protein synthesis inhibition, which in turn causes cell growth arrest. Consequently, each compound that inhibits any of the aa-RS could be a potential antibacterial agent. Only one aa-RS inhibitor, the Ile-RS inhibitor mupirocin, is currently marketed as an antibacterial agent. We focused on phenylalanyl (Phe)-tRNA synthetase (Phe-RS), but the described methods are not restricted to Phe-RS and might be adapted to other aa-RS.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bactérias
/
Inibidores Enzimáticos
/
Aminoacil-tRNA Sintetases
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article