A dynamic expression pattern of sGalpha(i2) protein during early period of postnatal rat brain development.
Int J Dev Neurosci
; 26(6): 611-24, 2008 Oct.
Article
em En
| MEDLINE
| ID: mdl-18472243
ABSTRACT
The function of sGalphai2 protein in central nervous system is not well understood. Therefore to explore the possible role of this protein in postnatal brain development, we have analyzed the protein expression pattern of brain obtained from rats of postnatal day 0 (P0) to P90 by dot-blots and immunocytochemistry techniques. In dot-blots, both nuclear and membrane fractions showed a gradual decrease from P0 to P60. Highest protein level was observed at the age of P0. There was also a trend of decline in the sGalphai2 protein from P0 to P90 in brain sections stained by immunocytochemistry method. At P0, the protein labeling was highest in cerebral cortex, hippocampus, cerebellum and mitral cell layer. In cerebral cortex, a drop in the immunolabeling of sGalphai2 protein was observed at P3, which was significantly increased at the age of P5. However, in striatum and olfactory tubercle, it was maintained through P0-P10 and P0-P5, respectively. Thalamus was one of the areas where labeling was not as strong as cortex, hippocampus or striatum. In contrary to other areas, immunostaining of sGalphai2 in corpus-callosum and lacunosum-molecular was not seen at P0 and appeared in advanced postnatal ages. A detectable level of sGalphai2 protein was observed at P5 in carpus-callosum and at P20 in lacunosum-molecular. A high level of sGalphai2 protein in the period when cellular layer organization and synaptic innervations, synaptic connections and maturation take place, suggests for a potential role of this protein in the early postnatal brain development.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Encéfalo
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Regulação da Expressão Gênica no Desenvolvimento
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Subunidade alfa Gi2 de Proteína de Ligação ao GTP
Limite:
Animals
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Pregnancy
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article