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Polyvalent display of heme on hepatitis B virus capsid protein through coordination to hexahistidine tags.
Prasuhn, Duane E; Kuzelka, Jane; Strable, Erica; Udit, Andrew K; Cho, So-Hye; Lander, Gabriel C; Quispe, Joel D; Diers, James R; Bocian, David F; Potter, Clint; Carragher, Bridget; Finn, M G.
Afiliação
  • Prasuhn DE; Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.
Chem Biol ; 15(5): 513-9, 2008 May.
Article em En | MEDLINE | ID: mdl-18482703
The addition of a hexahistidine tag to the N terminus of the hepatitis B capsid protein gives rise to a self-assembled particle with 80 sites of high local density of histidine side chains. Iron protoporphyrin IX has been found to bind tightly at each of these sites, making a polyvalent system of well-defined spacing between metalloporphyrin complexes. The spectroscopic and redox properties of the resulting particle are consistent with the presence of 80 site-isolated bis(histidine)-bound heme centers, comprising a polyvalent b-type cytochrome mimic.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligopeptídeos / Vírus da Hepatite B / Capsídeo / Heme / Histidina Idioma: En Ano de publicação: 2008 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligopeptídeos / Vírus da Hepatite B / Capsídeo / Heme / Histidina Idioma: En Ano de publicação: 2008 Tipo de documento: Article