Molecular cloning of PC3, a putatively secreted protein whose mRNA is induced by nerve growth factor and depolarization.
Proc Natl Acad Sci U S A
; 88(8): 3353-7, 1991 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-1849653
ABSTRACT
PC3 is an immediate early gene induced by nerve growth factor in PC12 cells, a cell line derived from a tumor of the adrenal medulla that undergoes neuronal differentiation in the presence of nerve growth factor. This induction is independent of new protein synthesis as it can occur in the presence of cycloheximide. PC3 is also induced with similar kinetics, but at lower levels, by membrane depolarization (both in vivo and in vitro) and epidermal growth factor. It is induced at much lower levels by fibroblast growth factor and interleukin 6. In vivo it is found expressed in tissues, such as brain at embryonic day 13.5, placenta, amnion, and spleen, which are proliferating and/or differentiating. The deduced protein sequence from the cDNA indicates the presence of a signal peptide, suggesting that PC3 is secreted.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Feocromocitoma
/
Neoplasias das Glândulas Suprarrenais
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Proteínas Imediatamente Precoces
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Potenciais da Membrana
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Proteínas de Neoplasias
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Fatores de Crescimento Neural
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Proteínas do Tecido Nervoso
Limite:
Animals
Idioma:
En
Ano de publicação:
1991
Tipo de documento:
Article