[Development of a SPA-ELISA method for detecting anti-coronavirus IgG antibodies in serum samples from fulvous fruit bats].
Nan Fang Yi Ke Da Xue Xue Bao
; 28(5): 736-8, 2008 May.
Article
em Zh
| MEDLINE
| ID: mdl-18504193
ABSTRACT
OBJECTIVE:
To develop an simple and sensitive method for detecting anti-coronavirus IgG antibodies in bat sera based on enzyme-linked immunosorbent assay (ELISA).METHODS:
A commercial ELISA kit for detecting SARS-CoV antibody was modified for detecting coronavirus antibodies in bat serum samples. The second antibody in the kit was replaced with horseradish peroxidase-conjugated protein-A (HRP-SPA) based on the characteristics of binding between Staphylococcus aureus protein A (SPA) and mammal IgG Fc fragment. The sera of 55 fulvous fruit bats (Rousettus dasymallus) were tested using the SPA-ELISA.RESULTS:
The test results of the positive and negative controls in the kit and the serum samples from convalescent ;patient were consistent with expectation. Coronavirus antibody was detected in 2 out of the 55 bat serum samples. Serum neutralization test confirmed the validity of the SPA-ELISA method.CONCLUSION:
This SPA-ELISA method is applicable for detecting coronavirus antibody in bat sera.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imunoglobulina G
/
Quirópteros
/
Coronavirus
/
Anticorpos Antivirais
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2008
Tipo de documento:
Article