[Up-regulation of CD86 and cytokine expression in acute leukemia cells by Ara-C].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
; 24(6): 550-2, 2008 Jun.
Article
em Zh
| MEDLINE
| ID: mdl-18538080
ABSTRACT
AIM:
To observe the effects of Ara-c on the expression of CD86 molecule on acute leukemia cells and explore the possible mechanisms.METHODS:
The expression of CD86 on U937, HL-60 and NB4 cell lines treated with or without Ara-C wa assayed by flow cytometry. The mRNA expression of CD86, NF-kappaB, IFN-gamma was examined by semiquantitative RT-PCR.RESULTS:
UP-regulation of CD86 was observed on those cells treated by Ara-c. The level of CD86 and NF-kappaB mRNA in Ara-c treated cells was significantly enhanced. IFN-gamma was detectable 72 hours after T cell activation.CONCLUSION:
Ara-C can enhance CD86 and NF-kappaB expression on acute leukemia cells, which may play critical role in T cell activation and differentiation.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Leucemia
/
Regulação para Cima
/
Citocinas
/
Citarabina
/
Antígeno B7-2
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2008
Tipo de documento:
Article