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CRHR1-dependent effects on protein expression and posttranslational modification in AtT-20 cells.
Kronsbein, Helena C; Jastorff, Archana M; Maccarrone, Giuseppina; Stalla, Günter; Wurst, Wolfgang; Holsboer, Florian; Turck, Christoph W; Deussing, Jan M.
Afiliação
  • Kronsbein HC; Max Planck Institute of Psychiatry, Kraepelinstrasse 2-10, D-80804 Munich, Germany.
Mol Cell Endocrinol ; 292(1-2): 1-10, 2008 Sep 24.
Article em En | MEDLINE | ID: mdl-18582531
Corticotropin-releasing hormone (CRH) plays a major role in coordinating the organism's stress response, including the activity of the hypothalamic-pituitary-adrenocortical axis. The molecular underpinnings of CRH-dependent signal transduction mechanisms in the anterior pituitary have not yet been revealed in detail. In order to dissect the signal transduction cascades activated by CRH receptor type 1, a comparative proteome approach was performed in vitro utilizing murine corticotroph AtT-20 cells. Alterations in protein expression and posttranslational modification in response to CRH stimulation were studied by 2D gel electrophoresis. Selected candidates were analyzed by immunoblotting and quantitative real-time PCR. The differential analyses revealed proteins regulated or modified related to diverse cellular processes. Amongst others we identified alterations in PRKAR1A, the regulatory subunit of protein kinase A; in PGK1 and PGAM1, key regulators of glycolysis; and in proteins involved in proteasome-mediated proteolysis, PSMC2 and PSMA3. These results offer novel entry points to molecular mechanisms underlying stress responses elicited via the hypothalamic-pituitary-adrenocortical axis.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas / Processamento de Proteína Pós-Traducional / Receptores de Hormônio Liberador da Corticotropina Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2008 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas / Processamento de Proteína Pós-Traducional / Receptores de Hormônio Liberador da Corticotropina Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2008 Tipo de documento: Article