Production and characterisation of a monoclonal antibody to progesterone and its effect on fertility.
Indian J Exp Biol
; 29(1): 6-11, 1991 Jan.
Article
em En
| MEDLINE
| ID: mdl-1864623
ABSTRACT
A monoclonal antibody reacting with progesterone has been raised by fusion of mouse myeloma cells (SP20) and splenocytes of BALB/c mice hyperimmunized with 4-pregnane 3,20 dione conjugated to bovine serum albumin. Association constant of this antibody for binding with progesterone was 0.22 x 10(9) l/mole. The antibody was highly specific for progesterone. A single ip injection of this antibody brought about an antifertility effect which is influenced by genotype. Antibody treatment brought about a significant decrease in the fetal weight and a slight decrease in the plasma progesterone levels. The antifertility effect could be reversed only up to day 3 by exogenous administration of progesterone.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Progesterona
/
Anticorpos Monoclonais
Limite:
Animals
Idioma:
En
Ano de publicação:
1991
Tipo de documento:
Article