Temperature-regulated activity of responsive polymer-protein conjugates prepared by grafting-from via RAFT polymerization.
J Am Chem Soc
; 130(34): 11288-9, 2008 Aug 27.
Article
em En
| MEDLINE
| ID: mdl-18665597
ABSTRACT
A facile route to well-defined "smart" polymer-protein conjugates with tunable bioactivity is reported. Protein modification with a reversible addition-fragmentation chain transfer (RAFT) agent and subsequent room temperature polymerization in aqueous media led to conjugates of poly(N-isopropylacrylamide) and a model protein. Representing the first example of polymer-protein conjugation with RAFT agent immobilization via the "R-group" approach, high molecular weight and reductively stable conjugates were accessible without extensive purification or adverse effects on the protein structure. An increase in molecular weight with conversion was observed for the chains grafted from the protein surface, confirming the controlled nature of the polymerization. The responsive behavior of the immobilized polymer facilitated conjugate isolation and also allowed environmental modulation of bioactivity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Materiais Biocompatíveis
/
Resinas Acrílicas
/
Proteínas
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article