A commercial ELISA detects high levels of human H5 antibody but cross-reacts with influenza A antibodies.
J Clin Virol
; 43(2): 241-3, 2008 Oct.
Article
em En
| MEDLINE
| ID: mdl-18675584
ABSTRACT
BACKGROUND AND OBJECTIVES:
Commercial serological assays to determine influenza A H5N1 infection are available, although the accuracy and reproducibility of these are not reported in detail. This study aimed to assess the validity of a commercial ELISA H5 hemagglutinin (HA) antibody kit. STUDYDESIGN:
A commercial ELISA for detection of antibodies towards influenza A H5 HA was evaluated using human sera from vaccinated individuals. The ELISA was used to screen 304 sera with elevated influenza A complement fixation titres collected between the period 1995-2007. RESULTS ANDCONCLUSIONS:
The ELISA was found to be accurate for sera with high levels of anti-H5 antibodies, and would be useful in clinical settings where a rapid result is required. Thirteen of the stored sera were positive using the ELISA, but were confirmed as negative for H5N1 exposure using further serological tests. Absorption studies suggested that antibodies towards seasonal H3N2 and H1N1 influenza may cross-react with H5 antigen, giving false positive results with the ELISA.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ensaio de Imunoadsorção Enzimática
/
Glicoproteínas de Hemaglutininação de Vírus da Influenza
/
Influenza Humana
/
Anticorpos Antivirais
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Adolescent
/
Adult
/
Aged
/
Aged80
/
Animals
/
Humans
/
Middle aged
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article