Whole blood as an alternative to plasma for detection of hepatitis C virus RNA.
J Clin Microbiol
; 46(11): 3791-4, 2008 Nov.
Article
em En
| MEDLINE
| ID: mdl-18701664
ABSTRACT
Peripheral blood mononuclear cells are reported to be one of the extrahepatic replication sites contributing to the persistence of hepatitis C virus (HCV) infection. Whole-blood and plasma samples from 61 individuals were compared as sources for the detection of HCV RNA. Forty-four of the individuals were receiving antiviral therapy, while 17 were treatment naïve. The quantitation of HCV RNA was done by a sensitive in-house real-time reverse transcription-PCR. When the viral loads in the two types of samples were compared, a correlation coefficient of 0.858 (P < 0.001) was found, indicating that plasma and whole blood are equally acceptable sources for testing for HCV RNA.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sangue
/
RNA Viral
/
Hepacivirus
/
Carga Viral
Tipo de estudo:
Diagnostic_studies
Limite:
Adolescent
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Adult
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Aged
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Female
/
Humans
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Male
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Middle aged
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article