The fusing ability of sperm is bestowed by CD9-containing vesicles released from eggs in mice.
Proc Natl Acad Sci U S A
; 105(35): 12921-6, 2008 Sep 02.
Article
em En
| MEDLINE
| ID: mdl-18728192
ABSTRACT
Membrane fusion is an essential step in the encounter of two nuclei from sex cells-sperm and egg-in fertilization. However, aside from the involvement of two molecules, CD9 and Izumo, the mechanism of fusion remains unclear. Here, we show that sperm-egg fusion is mediated by vesicles containing CD9 that are released from the egg and interact with sperm. We demonstrate that the CD9(-/-) eggs, which have a defective sperm-fusing ability, have impaired release of CD9-containing vesicles. We investigate the fusion-facilitating activity of CD9-containing vesicles by examining the fusion of sperm to CD9(-/-) eggs with the aid of exogenous CD9-containing vesicles. Moreover, we show, by examining the fusion of sperm to CD9(-/-) eggs, that hamster eggs have a similar fusing ability as mouse eggs. The CD9-containing vesicle release from unfertilized eggs provides insight into the mechanism required for fusion with sperm.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Óvulo
/
Interações Espermatozoide-Óvulo
/
Espermatozoides
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Glicoproteínas de Membrana
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Antígenos CD
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Vesículas Secretórias
Limite:
Animals
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article